By Emerson Perin, James T. Willerson
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Additional info for An Essential Guide to Cardiac Cell Therapy
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This test is conducted by injecting ESCs derived from the inner cell mass of one blastocyst into the cavity of another blastocyst. The resulting combination embryos are then implanted in the uterus of a female mouse, and the progeny that result are known as chimeras. Chimeras are a mixture of tissues and organs derived from both the recipient blastocyst and donor ESCs. If the resulting chimeras are complete embryos, then the donor stem cells must have been pluripotent. Cultured ESCs can also replace the inner cell mass of a mouse blastocyst and produce a normal embryo, but the process is far less efficient than that of using cells taken directly from the inner cell mass.