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A Text-Book of Medical Bacteriology by R. W. Fairbrother (Auth.)

By R. W. Fairbrother (Auth.)

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Extra resources for A Text-Book of Medical Bacteriology

Sample text

In order to indicate the presence of anaerobiosis a weak aqueous solution of méthylène-blue is added ; this in the absence of oxygen becomes colourless. , plates and tubes, may be grown under such conditions. A useful modification of the Mclntosh and Fildes' jar is t h a t by Wilson. This jar is constructed of a copper alloy, and consequently can stand much greater strains than the glass jar. Air is first removed by means of a vacuum pump and hydrogen is then slowly added ; an electric current is then passed through.

Cultures are prepared on solid media in Petri dishes by streaking. The platinum loop is charged with material and a series of strokes made on the surface of the medium. The most satisfactory method of thinning the bacterial growth is shown in Pig. 8. The first strokes are made at the side 1, the plate is rotated and further streaks made, starting at position 2, the plate is again rotated to 3 and a final series of strokes made. 44 A TE XT-BOOK OF MEDICAL BACTERIOLOGY I t is important that the surface of the medium should be dry, otherwise growth tends to be confluent.

The explanation of the various oxygen requirements of the different bacteria is uncertain. I t has been suggested that, in the case of the anaerobes, the atmospheric oxygen does not itself exert an injurious action on the organisms, but t h a t it is used up during bacterial growth to form hydrogen peroxide, to which these organisms are very susceptible. The aerobes, on the other hand, are resistant to H 2 0 2 , as in the case of the pneumococcus, or if susceptible produce a catalase, which breaks it down into water and oxygen.

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